Fractionation of Plasminogen Activator and Proactivator in Tissue and Blood by Gel Filtration.

Human plasma: Dried human plasma (Nihon Seiyaku Co., Ltd.) was used. Commercial preparations of fibrinogen (Cohn's Fraction I, Armour Labora tory), thrombin (Mochida Co.) and streptokinase (Varidase, Lederle Lab. of American Cyamid Co.) were used in the following experiments. Plasminogen (Kline's preparation) was kindly donated by A .B. KABI. Urea solution of 0.4 M and 0.04 M, and the soltion of 0.1 M Tris-HC1 (pH 8.0) in 0.2 M NaCl were used as eluants for gel filtration. Gel filtration method : This was principally the same as the method reported by Flodin et al(s). Sephadex G-200 (A.B . Pharmacia) was prepared by swelling for 24 hours in an excess of 0.4 M urea solution or 0.1 M Tris buffer. The column had the dimensions 2 by 8 or 2 by 30 cm respectively . After the gel was packed in the column, it was washed and saturated by urea solution or the buffer solution overnight. A sample was slowly injected on the gel bed by means of a syringe with a 1 mm polyethylene tube which had been carefully inserted through the solution down to level a few milimeters above the gel bed . In the case of euglo bulin solution or plasminogen solution, the sample was placed directly on the gel surface after taking out the buffer solution remaining over the gel . Elution was